Differentiation of Minute Virus of Mice and Mouse Parvovirus by High Resolution Melting Curve Analysis.

Dan Rao,Miaoli Wu,Jing Wang,Wen Yuan,Yujun Zhu,Feng Cong,Fengjiao Xu,Yuexiao Lian,Bihong Huang,Qiwen Wu,Meili Chen,Yu Zhang,Ren Huang,Pengju Guo

Journal of Virological Methods（2017）

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摘要

•A simple, sensitive, specific PCR-HRM assay was established for murine parvovirus.•The method differentiates MVM and MPV strains in one reaction tube.•Tissue samples and co-infection assays were tested to verify the method.

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关键词

MVM,MPV,VP2 gene,PCR,HRM

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