A simple and rapid DNA extraction method for Chlamydia trachomatis detection from urogenital swabs.

Matthew A Butzler,Jennifer L Reed,Sally M McFall

Diagnostic Microbiology and Infectious Disease（2017）

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摘要

A highly sensitive and specific Chlamydia trachomatis (CT) diagnostic test was developed by combining filtration isolation of nucleic acid (FINA) extraction with quantitative polymerase chain reaction including an internal control to identify test inhibition. A pilot study of 40 clinical specimens yielded 100% sensitivity and specificity.

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关键词

FINA,CT,HPR,IC,qPCR,Cq,CI,SP

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