Directed differentiation and long-term maintenance of epicardial cells derived from human pluripotent stem cells under fully defined conditions

Xiaoping Bao,Xiaojun Lian,Tongcheng Qian,Vijesh J Bhute,Tianxiao Han,Sean P Palecek

NATURE PROTOCOLS（2017）

引用 34|浏览22

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摘要

This protocol differentiates hPSCs into self-renewing epicardial cells by appropriate differentiation-stage-specific application of Gsk3 inhibitor, Wnt inhibitor, and then Gsk3 inhibitor again in a completely defined, xeno-free system.

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关键词

Cardiovascular models,Cell culture,Heart development,Stem-cell differentiation,Life Sciences,general,Biological Techniques,Analytical Chemistry,Microarrays,Computational Biology/Bioinformatics,Organic Chemistry

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