One-step reverse transcription loop-mediated isothermal amplification assay for detection of Apple chlorotic leaf spot virus.

•A set of four primers were designed for ACLSV based on conserved sequences from an alignment of the coat protein gene sequences for detection by RT-LAMP using total RNA extracted from ACLSV-infected leaves.•Optimized temperature and time for ACLSV detection by RT-LAMP were determined to be 64°C and 75min.•Successful application of RT-LAMP to field-collected apple samples demonstrated its potential broader application for effective disease diagnosis, thus to control ACLSV from further spread.