A biochip-based combined immunoassay for detection of serological status of Borrelia burgdorferi in Lyme borreliosis.

•This study introduced dithiobis (succinimidyl undecanoate) modified gold surface as a combined immunoassay platform;•Concurrent assessment enables clinical diagnosis and therapy strategy-making more efficiently;•The multiplex immunobiochip assay conferred increased overall sensitivity of seroprevalences of IgM or IgG antibody;•Detection limits of IgG antibody on the biochips were as little as microgramme level;•The both results for real sample tests were in consistency in both ELISA assay and protein biochip.