Clostridium difficile chimeric toxin receptor binding domain vaccine induced protection against different strains in active and passive challenge models.

•A chimeric fusion protein vaccine protects against C. difficile infection (CDI).•Tetravalent toxin vaccine is effective against hypervirulent C. difficile.•The vaccine was used to produce hyperimmune human antitoxin IgG in transgenic bovine.•Human antitoxin IgG has potential to treat and prevent recurrent CDI.