DNA amplification fingerprinting (DAF) applied to the investigation ofAcinetobacter baumanii isolated from intensive care unit patients
Irish Journal of Medical Science(1996)
摘要
Acinetobacter are important emerging nosocomial pathogens. In this paper thirteenAcinetobacter baumanii from intensive care patients were isolated. These were initially typed using the API-20 NE biotyping system and antibiogram
analysis. Results obtained using these methods failed to convincingly characterise the organisms. In this report a method
to characterise theseAcinetobacter baumanii isolates is described, which utilises a modified polymerase chain reaction (PCR), capable of generating genomic fingerprints,
known as DNA amplification fingerprinting (DAF). Purified chromosomal DNA of cultured clinical isolates ofAcinetobacter baumanii were subjected to DAF using the M13 universal sequencing primer. Polymorphic DNA bands produced, were visualised after agarose
gel electrophoresis and ethiduim bromide staining. Results demonstrated that six of the thirteen clinical isolates represented
one group and a second group of two isolates displayed identical fingerprint patterns. The remaining four organisms were all
unique. This genotype based method is rapid, simple, reproducible and may have potential as a means of specifically typingAcinetobacter spp. allowing the route(s) of nosocomial transmission to be identified and to assess the efficiency of instituted infection control
measures.
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关键词
Phage Typing, Acinetobacter Calcoaceticus, Plasmid Profile, Acinetobacter Baumanii, Modify Polymerase Chain Reaction
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