Identification of the ETT2 locus in human diarrheagenic Escherichia coli by multiplex PCR

Kayo Osawa, Mariko Shibata, Yoko Nishiyama,Manabu Kurokawa,Go Yamamoto,Shohiro Kinoshita, Nobumasa Kataoka

Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy（2006）

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摘要

An Escherichia coli type III secretion system 2 (ETT2) locus was discovered in enterohemorrhagic E. coli O157:H7. To determine presence or absence of the ETT2 locus in diarrheagenic E. coli , a multiplex polymerase chain reaction (PCR) encoding Shiga toxins ( stx 1 and stx 2 ), intimin ( eaeA ), and ETT2 ( etrA ) was developed for rapid detection. The ETT2 locus was identified not only in Shiga toxin-producing E. coli (STEC) but also in various non-STEC.

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关键词

ETT2,Enterohemorrhagic Escherichia coli,Diarrheagenic Escherichia coli,Multiplex PCR

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