Precise and efficient scarless genome editing in stem cells using CORRECT

Dylan Kwart,Dominik Paquet,Shaun Teo,Marc Tessier-Lavigne

NATURE PROTOCOLS（2017）

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摘要

CORRECT is a method comprising two variants, re-Guide and re-Cas, that enables efficient and scarless CRISPR/Cas9-based genome editing in hPSCs by introducing blocking mutations that hamper the re-editing of modified loci by Cas9.

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关键词

CRISPR-Cas9 genome editing,Induced pluripotent stem cells,Genetic engineering,Mutagenesis

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