Correlated fluorescence microscopy and cryo-electron tomography of virus-infected or transfected mammalian cells

Cheri M Hampton,Joshua D Strauss,Zunlong Ke,Rebecca S Dillard,Jason E Hammonds,Eric Alonas,Tanay M Desai,Mariana Marin, Rachel E Storms,Fredrick Leon,Gregory B Melikyan,Philip J Santangelo,Paul W Spearman,Elizabeth R Wright

NATURE PROTOCOLS（2016）

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摘要

Correlative light and electron microscopy (CLEM) promises new insight into cellular processes by combining spatiotemporal information with high-resolution structural information. This protocol describes cryo-CLEM of virus-infected mammalian cells.

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关键词

Cryoelectron tomography,Virus-host interactions,Cryoelectron microscopy,Fluorescence imaging

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