Analysis of RNA structure using small-angle X-ray scattering.

William A Cantara,Erik D Olson,Karin Musier-Forsyth

Methods（2017）

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摘要

•SAXS is ideal for RNAs that are intractable for crystallography, NMR and cryo-EM.•Non-canonical synthetase functions include recognition of tRNA-like elements.•Studying tRNA-like elements by SAXS requires rigorous sample preparation.•Preparation of RNA and RNA-protein complexes for SAXS studies.•Principles for accurate analysis of SAXS data are presented.

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关键词

SAXS,RNA structure,Aminoacyl-tRNA synthetase,Size-exclusion chromatography,tRNA-like structures,Native PAGE

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