Particle-based N-linked glycan analysis of selected proteins from biological samples using nonglycosylated binders.

•A method capable of multiplexed analyses of N-glycan profiles of specific proteins has been developed.•Streptavidin-coated magnetic beads are used in combination with nonglycosylated VHH antibody fragments.•Enzymatic deglycosylation and desialylation are performed on the beads using PNGase F and neuraminidase.•A simple clean-up step using porous graphitic carbon spin tips is performed before MALDI-TOF-MS analysis.•The glycosylation patterns of alpha-1-antitrypsin and transferrin in human serum and cerebrospinal fluid has been measured.