Multiperspective smFRET reveals rate-determining late intermediates of ribosomal translocation

Michael R Wasserman,Jose L Alejo,Roger B Altman,Scott C Blanchard

NATURE STRUCTURAL & MOLECULAR BIOLOGY（2016）

引用 93|浏览9

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摘要

Monitoring ribosomal translocation from five structural perspectives with pre–steady state smFRET defines intramolecular conformational events within the EF-G–GDP–bound ribosome as rate-determining steps of directional substrate translocation.

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关键词

Single-molecule biophysics,Ribosome,RNA

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