A LC-MS–based workflow for measurement of branched fatty acid esters of hydroxy fatty acids

Tejia Zhang,Shili Chen,Ismail Syed,Marcus Ståhlman,Matthew J Kolar,Edwin A Homan,Qian Chu,Ulf Smith,Jan Borén,Barbara B Kahn,Alan Saghatelian

Nature Protocols（2016）

引用 58|浏览1

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摘要

Branched fatty acid esters of hydroxy fatty acids (FAHFAs) are a recently discovered class of biological lipids. This protocol describes their extraction from serum and tissue samples, followed by enrichment and analysis by LC-MS.

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关键词

Bioanalytical chemistry,Lipids,Liquid chromatography,Mass spectrometry,Life Sciences,general,Biological Techniques,Analytical Chemistry,Microarrays,Computational Biology/Bioinformatics,Organic Chemistry

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