Real-time monitoring of basal H 2 O 2 levels with peroxiredoxin-based probes
NATURE CHEMICAL BIOLOGY(2016)
摘要
Editorial Summary A fluorescent sensor combining a mutated form of the 2-Cys peroxiredoxin Tsa2 unable to undergo thioredoxin-mediated reduction with a redox-sensitive GFP protein allows real-time detection of baseline hydrogen peroxide levels in yeast cells.
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关键词
Imaging,Biochemistry,Chemical tools,Metabolism
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