Nanoscale imaging of RNA with expansion microscopy

Fei Chen,Asmamaw T Wassie,Allison J Cote,Anubhav Sinha,Shahar Alon,Shoh Asano,Evan R Daugharthy,Jae-Byum Chang,Adam Marblestone,George M Church,Arjun Raj,Edward S Boyden

NATURE METHODS（2016）

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摘要

ExFISH extends expansion microscopy to single-molecule RNA imaging, enabling super-resolution imaging of diverse RNAs in cells and tissues on conventional microscopes. The method enables multiplexed imaging of RNA and improved RNA quantitation.

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关键词

Fluorescence imaging,Super-resolution microscopy,Molecular neuroscience,Fluorescence in situ hybridization

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