Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro

Jérome Chal,Ziad Al Tanoury, Marie Hestin, Bénédicte Gobert, Suvi Aivio, Aurore Hick,Thomas Cherrier,Alexander P Nesmith,Kevin K Parker,Olivier Pourquié

Nature Protocols（2016）

引用 194|浏览2

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摘要

This protocol uses dual modulation of Wnt and BMP signaling pathways to differentiate human pluripotent stem cells into striated, millimeter-long muscle fibers and satellite-like cells in vitro without the need to introduce genetic material or cell sorting.

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关键词

Stem cells,Musculoskeletal models,Stem-cell differentiation,Cell culture

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