Expression microdissection isolation of enriched cell populations from archival brain tissue.

C Appleby-Mallinder,M D Wyles,J E Simpson,S B Wharton,P G Ince,P R Heath

Journal of Neuroscience Methods（2016）

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摘要

•Expression microdissection (xMD) reduces issues of laser capture microdissection.•xMD reduces procedure length compared to LCM.•xMD can isolate immunopositive central nervous system cells (CNS) from FFPE tissue.•RNA integrity number suggests RNA is of sufficient quality for further analysis.•CNS cells isolated by xMD show enriched populations, confirmed by RT-PCR analysis

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关键词

CNS,LCM,EVA,DAB,xMD,FFPE,RT-PCR,ABC,DEPC,TBS,UV,RIN

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