2SNV: Quasispecies reconstruction from PacBio reads
ICCABS(2015)
摘要
Pacific Biosciences (PacBio) sequencing is providing thousands of reads with the length up to 10,000 bases. In most cases this length is enough to cover entire region of interest however this technology has high (approximate to 15%) error rate. We propose a method for viral haplotype reconstruction using long SMRT reads. The proposed method based on correlation between SNVs and maximum likelihood for frequency estimation. When applied to PacBio reads from an Influenza A Virus (IAV) sample with ten variants, our method was able to reconstruct the nine most frequent.
更多查看译文
关键词
PacBio, viral quasispecies, SNV
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络