Elevation Of Non-Classical (Cd14(+/Low)Cd16(++)) Monocytes Is Associated With Increased Albuminuria And Urine Tgf-Beta(1) In Hiv-Infected Individuals On Stable Antiretroviral Therapy

ObjectiveHigh rates of albuminuria are observed among HIV-infected individuals on stable antiretroviral therapy (ART). Though pro-inflammatory and pro-fibrotic responses are described as components of albuminuria in the general population, it is unclear how these responses are associated to albuminuria in ART-treated chronic HIV. We investigated the relationship of monocyte subsets and urine inflammatory and fibrotic biomarkers to albuminuria in ART-treated HIV-infected participants.Design and MethodsCross-sectional analyses were performed on Hawaii Aging with HIV-cardiovascular disease study cohort participants who were required at entry to be >= 40 years old and on ART >= 3 months. Monocyte subpopulations were determined in banked peripheral blood mononuclear cells (PBMC) using multi-parametric flow-cytometry. Entry random urine samples were assessed for albumin-to-creatinine ratios (UACR). Urine samples were measured for inflammatory and fibrotic biomarkers using Luminex technology.ResultsAmong 96 HIV-infected subjects with measured UACR (87% male, 59% Caucasian, and 89% undetectable HIV RNA with median CD4 of 495.5 cells/mu L), 18 patients (19%) had albuminuria. Non-classical (CD14(low/+)CD16(++)) monocytes were significantly elevated in subjects with albuminuria (p = 0.034) and were correlated to UACR (r = 0.238, p = 0.019). Elevated non-classical monocyte counts were significant predictors of worsening albuminuria, independent of traditional-and ART-associated risk factors (beta = 0.539, p = 0.007). Urine TGF-beta 1 and collagen-IV were significantly higher in albuminuric compared to non-albuminuric participants (TGF-beta 1; p = 0.039 and collagen-IV; p = 0.042). Urine TGF-beta 1 was significantly correlated with non-classical monocyte counts (r = 0.464, p = 0.017).ConclusionAlterations in monocyte subpopulations and urine pro-fibrotic factors may play a role in kidney dysfunction during chronic HIV infection and warrants further study.