Quantitative analysis of Babesia ovis infection in sheep and ticks.

Oran Erster,Asael Roth,Ricardo Wollkomirsky,Benjamin Leibovich,Igor Savitzky,Shmuel Zamir,Thea Molad,Varda Shkap

Veterinary Parasitology（2016）

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摘要

•A quantitative real-time PCR assay for Babesia ovis detection was developed.•Different B. ovis load was detected in sheep infection experiment using the new assay.•B. ovis load in salivary glands of pre-fed ticks was assessed using the new assay.•Low-level parasitemia in naturally-infected flocks was assessed with the new assay.

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关键词

Babesia ovis,Rhipicephalus bursa,Quantitative PCR,BoSPD,Experimental infection

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