Elucidating the Key Determinants of Structure, Folding, and Stability for the () Conformation of the B1 Domain of Protein G Using Bioinformatics Approaches.

The B1 domain of protein G (GB1) is a small, 56 amino acid bacterial immunoglobulin-binding protein with a 4β + α fold. Architecturally, it is composed of a two-layer sandwich consisting of a four-stranded β-sheet that packs against an α-helix. Using several bioinformatics approaches, we investigated which residues may be key determinants of this fold. We identified nine structurally conserved ami...