Cell attachment and proliferation of osteoblast-like MG63 cells on silk fibroin membrane for guided bone regeneration
Maxillofacial plastic and reconstructive surgery(2016)
摘要
Background The aim of this study is to verify the feasibility of using silk fibroin (SF) as a potential membrane for guided bone regeneration (GBR). Methods Various cellular responses (i.e., cell attachment, viability, and proliferation) of osteoblast-like MG63 cells cultured on an SF membrane were quantified. After culturing on an SF membrane for 1, 5, and 7 days, the attachment and surface morphology of MG63 cells were examined by optical and scanning electron microscopy (SEM), cell viability was determined using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and cell proliferation was quantified using 4′,6-diamidino-2-phenylindole (DAPI) fluorescence staining. Results Optical microscopy revealed that MG63 cells cultured on the SF membrane proliferated over the 7-day observation period. The viability of cells cultured on SF membranes (SF group) and on control surfaces (control group) increased over time ( P < 0.05); however, at respective time points, cell viability was not significantly different between the two groups ( P > 0.05). In contrast, cell proliferation was significantly higher in the SF membrane group than in the control group at 7 days ( P < 0.05). Conclusions These results suggest that silk fibroin is a biocompatible material that could be used as a suitable alternative barrier membrane for GBR.
更多查看译文
关键词
Bone regeneration,Cell adhesion,Cell proliferation,Membrane,Osteoblasts,Silk fibroin
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要