Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes.

•The use of a canine protective vaccine would be an important control tool to canine leismaniosis.•The vaccination employing Leishmune® induces in neutrophils and monocytes increase in phagocytic activity and NO production.•The vaccination induces increased expression of TLR in neutrophils and monocytes from dogs.•The vaccination induces preferentially inflammatory profile of cytokines.