The glycolytic enzyme enolase represents a plasminogen-binding protein on the surface of a wide variety of medically important fungal species

•Recombinant enolases from fungal and human sources have been purified.•Monoclonal antibodies with fungal enolase specificity have been generated.•Monoclonal antibodies allow fungal species differentiation.•General surface presentation of fungal enolases is confirmed.•Surface enolases bind plasminogen that can still be activated.