RNA interference in adult Ascaris suum – an opportunity for the development of a functional genomics platform that supports organism-, tissue- and cell-based biology in a nematode parasite

•RNA interference (RNAi) is readily achievable in adult Ascaris suum.•RNAi is capable of spreading in adult A. suum.•Multiple A. suum tissue-specific targets are susceptible to RNAi.•Double-stranded RNA cocktails efficiently silence multiple targets simultaneously.•RNAi is consistent across geographical isolates of A. suum.