High Resolution Melting-Typing (HRMT) of methicillin-resistant Staphylococcus aureus (MRSA): The new frontier to replace multi-locus sequence typing (MLST) for epidemiological surveillance studies.

Gino Mongelli,Dafne Bongiorno, Marilena Agosta,Sabrina Benvenuto,Stefania Stefani,Floriana Campanile

Journal of Microbiological Methods（2015）

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摘要

We report an implemented molecular-typing-method based on HRMA to detect SNPs within MLST loci, characterizing 100 clinical MRSA and 11 control strains, representative of Italian clones.

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关键词

Methicillin-resistant Staphylococcus aureus (MRSA),High Resolution Melting Typing (HRMT),Single-nucleotide polymorphisms (SNPs),Molecular-epidemiology,Cost and time reduction

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