Extraction of Effective Compounds in Pine Pollen and In Vitro Assay of their Antitumor Effect

Supercritical CO2 was used to extract effective compounds from pine pollen, MTT colorimetric method was applied to determine anti-cancer effect of effective compounds in pine pollen. Results indicated that extracts obtained at pressure ranges 0 similar to 30 MPa and 10 similar to 15 MPa had significant inhibition effect on the lung cancer cells 95D of pig (95D) and their IC50 value was 253.22 mu g/mL and 252.19 mu g/mL, respectively. Extracts attained at pressure ranging 0 similar to 10 MPa, 10 similar to 15 MPa and 0 similar to 30 MPa had significant inhibition effect on the stomach cancer cells SGC-7901 of human (SGC-7901), and their IC50 value was 248.35 mu g/mL, 459.56 mu g/mL and 301.20 mu g/mL, individualy. Supercritical CO2 extract of pine pollen had inhibition effects on the lung cancer cells 95D of pig and the stomach cancer cells SGC-7901 of human, and the corresponding active compounds were different.