Determination of Aflatoxin Contaminants in Four South Medicines by HPLC

A method was developed for the simultaneous determination of Afiatoxin ( AFB1、AFB2、AFG1、AFG2) in four south medicines is developed by Liquid chromatograPhy with fluoreseence detection after immunoaffinity column clean一up and Pre一cloumn derivatization.four south medicine samples were extraeted with methanol一water (70:30,v/v),The extraets were Purified by imunoaffinity columns and the toxins were separated by reversed phase HPLC, and quantified with fluorescenee detection after photoehemieal derivatization. The separation was perfored on a C18 column.Recoveries of the different medicines spiked with Aflatoxin, The average recoveries ranged from 94.0 %~92.3 % by addition of avermectin standards at three different concentration levels of 10 μg/kg) to four south medicines as matrixes. The sensitivity, accuracy and precisiothod were able to meet the requirements for pesticide residue analysis.