Solar-to-bioH 2 production enhanced by homologous overexpression of hydrogenase in green alga Chlorella sp . DT

Green algae are able to convert solar energy into biological hydrogen (solar-to-H2) from H+ via chloroplast hydrogenase (HydA), which can accept electrons directly from ferredoxin and generates H2. HydA is nuclear-encoded by the hydA gene, and its transcription and activity can only be observed under anaerobic or sulfur-deprived conditions.