Abstract 1380:In vivoevidence for prior manipulation therapy-induced metastasis of primary osteosarcoma.
Cancer Research(2013)
摘要
Proceedings: AACR 104th Annual Meeting 2013; Apr 6-10, 2013; Washington, DC
Our previous clinical study demonstrated that manipulation therapy (MT) on osteosarcoma (OS) patients prior to diagnosis resulted in poor prognosis after surgical treatment. This study was aimed to provide in vivo evidence of MT-induced metastasis in primary osterosarcoma. Eight-week-old male GFP-labeled human OS cells-transferred nude mice were randomly allocated into 2 groups, namely, MT (+) and MT (-) groups. MT was conducted with repeated massage on tumor site twice a week for 7 or 15 weeks. The parameters evaluated were x-ray diagnosis, micro-PET/CT scan, histopathology and serum metalloproteinase 9 (MMP9) level. The results showed that MT (+) mice showed a decreased body weight (30.5±0.65g) and an increased tumor volume (8.3±1.18 mm3) compared to MT (-) group with body weight (35.8±0.40g, p<0.0001) and tumor volume (3.9±1.34mm3, p=0.038), respectively. There was an increased signal intensity over lymph node region of hind limb by micro-PET/CT and the GFP-labeled human OS cells were detected in the lung and bilateral lymph nodes in MT (+) group, while there were no such findings in MT (-) group. The serum MMP9 level was higher in MT (+) group (27.1±1.29 ng/ml) than in MT (-) group (17.8±1.97 ng/ml, p=0.048). Taken previous clinical observation and the present in vivo evidence together, we conclude that physicians should pay more attention on those patients who seek MT before diagnosis or during treatment for osteosarcoma.
Citation Format: Jir-You Wang, Po-Kuei Wu, Chih-Hsueh Chen, Chuen-Chuan Yen, Giun-Yi Hung, Shih-Chieh Hung, Wei-Ming Chen, Chien-Lin Liu, Tain-Hsiung Chen. In vivo evidence for prior manipulation therapy-induced metastasis of primary osteosarcoma. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 1380. doi:10.1158/1538-7445.AM2013-1380
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