Abstract 2132: Analysis of adenylate cyclase 6 in HMBA induced K562 cell megakaryocyte differentiation.

Proceedings: AACR 104th Annual Meeting 2013; Apr 6-10, 2013; Washington, DC Leukemia is a hematopoietic stem cell disorder and leukemia patients suffer their lost in functional blood cell and the ratio of non-function cell increased. K562 cell was isolated from the pleural effusion of the leukemia patient and was used as a model cell line to study the relationship between the blood cell differentiation and signal transduction. In this report, three different inducers, huangqi (Astragalus membranaceus) extract, chemicals Hemin and HMBA were used to induce K562 cell differentiation and several cluster of differentiation (CD) marker were used to identify the cell linage. Two megakaryocyte markers were up-regulated in these treatments. The CD61, a late marker, was highly expressed in HMBA treated cell and CD41, an earlier marker, was identified in huangqi and HMBA treated cell. In former report, the Gi2α and Gsα was induced by the HMBA administration. Since both G proteins affect their downstream effector, adenylate cyclases (ADCYs), the ADCYs that could be participated in the pathway were examined by RT-PCR. The ADCY1 and ADCY6 were up-regulated in the HMBA-induced K562 cell. The ADCY6 was further studied because it coupled to calcium ion channel. The recombinant ADCY6 transfected cells were treated with three inducers, the results showed that all inducers induceed CD61 expression especially in HMBA treated sample. Also, the CD61 gene was 25 times higher expression as the parental cell. The results imply that the signals activated from HMBA and ADCY6 together may participate in the megakaryocyte differentiation. The study suggested that K562 cells can change the cell destination through the ADCY isoforms expression and other signal genes. These results may be used as the reference of the drug discover for leukemia treatment. Citation Format: Shin Hua Jiang, Chiu Yi Ho, Tai Lin Lee, Yun Shiang Chang, Wei Tung Huang, Meng Feng Tsai, Ang Yuan. Analysis of adenylate cyclase 6 in HMBA induced K562 cell megakaryocyte differentiation. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 2132. doi:10.1158/1538-7445.AM2013-2132