Alpha-lipoic acid supplementation protects enzymes from damage by nitrosative and oxidative stress

•We identified new antioxidant protein targets for α-lipoic acid activity.•α-Lipoic acid supplementation restores mitochondrial enzymatic activities.•α-Lipoic acid improves ATP generation inhibited by nitrosative stress.•Our findings disclose a novel redox regulatory function of α-lipoic acid.•Our data suggest a novel strategy for treatment of inflammation-related diseases.