Ratiometric 3-D Scanning Cytometer for Quantifying Cell-Surface Biomarker Expression within Intact Tissues

G S Kino,Christopher H Contag,James M Crawford,Jonathan T Liu,Mike W Helms,Michael J Mandella

Ntm（2009）

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摘要

We have developed a two-color ratiometric microscopy technique that not only reduces image contrast due to nonspecific probe accumulation, but also provides a quantitative 3D map of cellular biomarker expression.

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