Purification, Characterization, and Crystallization of Membrane Bound E. coli Tyrosine Kinase

Protein Expression and Purification（2016）

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摘要

•Active, full length E. coli tyrosine kinase (Etk) can be expressed and purified.•Small molecule additives can improve the in vitro behavior of Etk.•Truncation of Etk enables crystallization of the periplasmic domain.•Detergent choice in crucial for protein stability and crystallization.

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关键词

E. coli tyrosine kinase,Membrane protein purification,Detergent,Crystallization

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