Expression And Purification Of HER2 Extracellular Domain Proteins In Schneider2 Insect Cells

Shanthi Kanthala,Christopher Mill,David J Riese,Mihir Jaiswal,Seetharama Jois

Protein Expression and Purification（2016）

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摘要

•HER2 protein extracellular domain and domain IV were expressed in S2 insect cells.•Proteins were secreted into the medium in fully folded form.•Proteins were purified and stability was analyzed by circular dichroism spectroscopy.•Sequence analysis was done by tandem mass spectrometry.•Sequence information was obtained from collision induced dissociation fragmentation.

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关键词

Human epidermal growth factor receptor 2,S2 insect cell lines,Protein sequencing,Mass spectrometry,Protein secretion,Disulfide loops

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