Structural characterization by transmission electron microscopy and immunoreactivity of recombinant Hendra virus nucleocapsid protein expressed and purified from Escherichia coli

Lesley A Pearce,Meng Yu,Lynne J Waddington,Jennifer A Barr,Judith A Scoble,Gary Crameri,William J Mckinstry

Protein Expression and Purification（2015）

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摘要

•Recombinant HeV N was expressed in a soluble from in E. coli.•HeV N purified by IMAC and SEC formed higher order oligomers.•Negative-stain EM images of recombinant HeV N indicated self-assembly to form helical chains of nucleocapsids.•Recombinant forms of HeV N were immuno-reactive with sera from infected animals and humans.

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关键词

CedPV,EIDs,ESI-TOF-MS,HeV,HeV N,HeV NFL,HeV NCORE,IDR,IMAC,IPTG,L,MERS-CoV,MeV,N-RNA,NiV,NiV N,N,nsNSRV,P,RNP,SARS-CoV,SDS–PAGE,SEC,sG,TBS,TEM

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