Molecular characterisation of bud necrosis virus isolates infecting tomato (Lycopersicon esculentum Mill.) by coat protein gene analysis in Andhra Pradesh

The complete nucleotide sequence was determined for coat protein (CP) gene of Groundnut bud necrosis virus (GBNV) infecting tomato in Andhra Pradesh (AP). The sequenced region in GBNV-To isolate contained a single open reading frame of 831 bases that could potentially code for a CP of 276 amino acids. The CP gene sequence of GBNV-To has 93–99% sequence homology with that of the gene sequences of other GBNV isolates prevalent on different hosts in India. In the present study, it is determined that the GBNV-To isolate of tomato prevalent in AP is regarded as the same strain of GBNV already existing in India.