Quantifying transgene flow rate in transgenic Sclerotinia-resistant peanut lines

•Gene flow from transgenic peanut lines to parents was measured in field trials.•The overall transgene flow rate was 0.2094% based on screening over 85,000 seeds.•Transgene flow rate greatly declined beyond 10m from transgene source.•<0.05% transgene flow rate did occur sporadically at greater distances than 10m.•At distance >10m, gene flow rate was dependent on foraging patterns of bees.