The Study of Media Effect on Differentiation of Gingival Fibroblast

Oral fibroblasts are the important cell in the repair of oral and maxillofacial tissues. This study examined whether in vitro the differentiation pattern of gingival fibroblast can be enhanced according to the cultivating media. The 4th passage gingival fibroblast cells were cultured in a medium containing Dulbecco's modified Eagle medium supplemented with 10% fetal bovine serum and 1 X antibiotic antimycotic solution. The cells was incubated at 37°C in 5% CO2 and 95% humidity, and the cultivating media was changed every 2 days. The control gingival fibroblast was cultured in a medium containing Dulbecco's modified Eagle medium for 3 months. The experimental group 1 gingival fibroblast was cultured in a medium containing Dulbecco's modified Eagle medium for a first stage, and cultured in a medium containing Minimum essential medium for a second stage. The experimental group 2 gingival fibroblast was cultured in a medium containing Minimum essential medium. Bone nodule-like material was observed control and experrimental groups. The experiment group 1 cultured in a medium containing Dulbecco's modified Eagle medium and Minimum essential medium showed a nodule-like shape more clearly than the control group. These results suggest that the nodule-like expression of gingival fibroblast can be increased according to the cultivating media, and that the oral fibroblast can be used for oral and maxillofacial tissue engineering.