The Study of Differentiation of Oral Fibroblast According to the Cultivating Space

The major cell in the gingival connective tissue is fibroblast. The major role of the fibroblast is repair of defect in the oral cavity. The differentiation study of oral fibroblast is important for oral and maxillofacial tissue engineering. The gingival fibroblasts was incubated at 37°C in 5% CO2 and 95% humidity, and the cultivating media was changed every 2 days. The oral fibroblast cells were cultured in a medium containing Dulbecco's modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum and 1 X antibiotic antimycotic solution. The control oral fibroblast was cultured in the wide cultivating space. The experimental oral fibroblast was cultured in the narrow cultivating space. After culturing of the fibroblasts, the cells was collected for immunohistochemicalstaing. The control cells was negative for S-100 immunohistochemical assay. The experimental cells was focal positive for S-100 immunohistochemical assay. Overall, these results suggest that the differentiation of oral fibroblast can be enhanced according to the cultivating space, and that the oral fibroblast is useful for oral and maxillofacial (chondroid etc.) tissue engineering.