Mo1735 Mechanism of Immune Regulation by CD11b+ Myeloid Cells in the Intestine

with 12 non-IBD family controls in EBV-transformed B cells.The mRNA expression levels of PTPN2 were increased in diseased tissues compared with adjacent normal tissues in 32 CD patients.The overall mean PTPN2/GAPDH expression values were: 4.36 ± 6.5 (diseased), vs. 2.45 ± 3.2 (normal) (p < 0.05).However, mRNA expression levels of PTPN2 were not significantly altered in the 30 UC diseased tissues vs adjacent normal tissues.Our previous cDNA microarray results showed that PTPN2 could be down-regulated by Nkx2-3 knockdown in B cells from CD and UC patients, and human intestinal microvascular endothelial cells (HIMECs).We confirm these results by RT-PCR in Nkx2-3 knockdown B cells from CD and UC, and Nkx2-3 knockdown HIMECs.The mRNA expression levels of Nkx2-3 were increased in 14 CD compared with 14 non-IBD family controls in EBV-transformed B cells (p < 0.05), and were increased in diseased tissues compared with adjacent normal tissues in 32 CD patients (p < 0.05).However, mRNA expression levels of Nkx2-3 were not significantly altered in EBV-transformed B cells and intestinal tissues from UC patients.mRNA expression levels of PTPN2 were positively correlated with those of Nkx2-3 in 14 CD (r = 0.6024, p < 0.01) and 12 UC (r = 0.5928, p < 0.01) from EBV-transformed B cells.Conclusions PTPN2 SNP rs2542152 was associated with CD. mRNA expression of PTPN2 was up-regulated in CD patients from EBV-transformed B cells and intestinal tissues.Expression of PTPN2 was regulated by Nkx2-3 in CD patients.