Optimum HPLC parameters for simultaneous determination of Robinin and Kaempferol

n HPLC method for simultaneous quantitative determination of robinin and its aglycone kaempferol in MCF-7 human breast carcinoma cell line was developed. Robinin and kaempferol were separated using a Platinum EPS C-18 column (4.6 × 250 nm, 5 μm particle size). The detector was set at 380 nm. The mobile phase consisted of solution A [5 % MeOH in phosphate buffer (0.01 M, pH 2.0)] and solution B (THF: i -PrOH:MeOH:H 2 O, 150:200:67.5:32.5) in a 60:40 ratio (A:B). Calibration curves for both compounds were linear ( R 2 = 0.9997, R 2 = 1) in the concentration range 25 – 1000 ng/mL. The intra- and interday variation coefficients were 1.31 – 8.92 %. The mean recoveries were 97.3 – 104.6 %. Robinin, a flavonoid glycoside isolated from leaves and flowers of Astragalus falcatus , decreases residual nitrogen, creatine, and urea in blood. The finished tablet dosage form prepared from it, flaronin, is used to treat uremia caused by chronic kidney failure.