Correction to “Comparative analysis of complete chloroplast genome sequence and inversion variation in Lasthenia burkei (Madieae, Asteraceae)”

Vol 101 No. 4: 722–729, April 2014, doi: 10.3732/ajb.1400049 In this article, we interpreted the alternative orientation of the small single copy (SSC) region in the plastomes of Artemesia frigida, Lactuca sativa and Nicotiana sylvestris relative to the other species in our analysis to be the result of inversion events. However, the SSC region has been shown to exist in two equimolar states within individual plants (Palmer, 1983), and thus the variation documented in our analysis is likely due to within-individual (rather than among-species) variation. The orientation of the SSC region is not standardized in GenBank accessions, and we included only one plastome per taxon in our analysis, so we misinterpreted within-species (and more specifically, within-individual) variation as among-species variation. Although Liu et al. (2013) attempted to confirm an inversion of the SSC region in Artemesia frigida using a PCR test, it has come to our attention that this test would also have mistaken the alternative states of the SSC region for inversion events. As a result of this realization, we would like to correct the conclusions in our paper that attributed the SSC variation observed among taxa to inversion events, rather than the likely correct explanation that this is due to alternative states of the SSC region within individual plants. Importantly, all conclusions of this study not associated with the orientation of the SSC region are not affected by this correction. This correction has the following impacts on the figures and tables in the manuscript: Figure 1: The line and label identifying “INV3” should be removed. Figure 2: The circles and labels identifying “INV3” should be removed. Figure 3: No species names should be in bold text. Table 1: The values corresponding to L. sativa, A. frigida, and N. sylvestris in the “IR regions” column should not be in bold text, and the column sub-header “Inversion site 3” should be “SSC region”. Supplementary Figure 1: The red box around the region between roughly 107kb and 127 kb should be removed.