Improved immobilization and stabilization of lipase from Rhizomucor miehei on octyl-glyoxyl agarose beads by using CaCl 2

Laura Fernandezlopez,Nazzoly Rueda,Rocio Bartolomecabrero,Maria Daniela Rodriguez,Tiago L Albuquerque,Jose C S Dos Santos,Oveimar Barbosa,Roberto Fernandezlafuente

Process Biochemistry（2016）

引用 68|浏览5

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摘要

•CaCl2 remained soluble at pH 10 in Gly buffer.•CaCl2 stabilized OC-RML but nor OC-CRL at pH 10.•OCGLX-RML prepared in presence of CaCl2 exhibited improved activity and stability.•CaCl2 and MnCl2 stabilized also OCGLX-RML biocatalysts.•OC-X-RML are also stabilized by CaCl2 in solvent inactivations.

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关键词

Lipase interfacial activation,Octyl-glyoxyl,Stabilizing additives,Lipase stabilization,Cations,Covalent immobilization

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