Coverage percentage and raman measurement of cross-tile and scaffold cross-tile based DNA nanostructures

•We present free-solution annealed DNA nanotubes and DNA nanolattices consisting of cross-tiles (CT1 and CT2) and a scaffold cross-tile (SCT).•When we perform mica-assisted growth annealing with CT1, a dimensional change occurs where the 1D nanotubes transform into 2D nanolattices.•We assessed the coverage percentage of the 2D nanolattices grown on the mica substrate as a function of the DNA concentration.•Finally, we fabricated divalent and trivalent ion-modified 2D nanolattices which were examined via Raman spectroscopy.