Dietary medium chain fatty acids from coconut oil have little effects on postprandial plasma metabolite profiles in rainbow trout ( Oncorhynchus mykiss )

This study examined the effect of dietary medium-chain triglycerides supplied by coconut oil on postprandial plasma metabolite profiles in rainbow trout. The fish (initial body weight 71.3±0.3g, 17°C) were fed one of four practical diets containing either 5% fish oil (FO low-fat, FL), 15% fish oil (FO high-fat, FH), 5% coconut oil (CO low-fat, CL) or 15% coconut oil (CO high-fat, CH) for 3weeks. At the end of the trial, the fish were weighed and plasma sampled to determine glucose, non-esterified fatty acids (NEFA), triglyceride (TG), cholesterol, high density lipoprotein-cholesterol (HDL-cholesterol), and myeloperoxidase (MPO) at 3, 6, 9, 12, 15 and 24h after the last meal. Plasma total ketone bodies (KB) were determined at 6, 12 and 24h after meal. Blood nitroblue tetrazolium (NBT) tests were also performed in samples withdrawn at 24h after meal.