Simultaneous Determination of Nelarabine and Its Active Metabolite 9-β- d -Arabinofuranosylguanine (Ara-G) in Human Plasma

A novel, simple and reliable bioanalytical method of high performance liquid chromatography for simultaneous quantification of nelarabine and its active metabolite 9-β- d -arabinofuranosyl-guanine (Ara-G) in human plasma was developed and validated. The calibration curves for nelarabine and Ara-G were linear over concentration ranges of 0.1562–10 and 0.6250–40 μg mL −1 , respectively. Intra- and inter-day precision and accuracy results satisfied the acceptable criteria for bioanalytical method validation. This method has been applied to a pharmacokinetic study involving 20 T-cell lymphoblastic leukemia/lymphoma patients.