UnusualHLA-DR/DQ haplotypes: two different breakpoints in two differentDR2-DQw3 haplotypes

Recently we had the opportunity to study a family from Bangkok in which unusual haplotypes, HLA-DRw15 (DR2) and DQw7 (DQw3), could be identified. The maternal haplotype is unusual in that it shows a combination of DRw15 and DQw7 (w3), which, to our knowledge, has thus far not been observed. To validate and extend the above findings, genomic DNA was prepared from peripheral blood of all family members and digested with the restriction enzymes Eco RI, Taq I, Pvu II, and Hind III