Partially purified malleo-proteins production for glanders diagnosis in Equidae

Silva K.P.C., Takaki G.M.C., Teles J.A.A., Dantas A.F.M., Costa M.M., Felix W.P. & Mota R.A. 2014. [Partially purified malleo-proteins production for glanders diagnosis in Equidae.] Producao e purificacao parcial de PPD-male ina para diagnostico do mormo em equi deos. Pesquisa Veterinaria Brasileira 34(1):57-61. Laboratorio de Doencas Infecciosas dos Animais, Faculdade de Veterinaria, Unidade Educacional Vicosa, Campus Arapiraca, Universidade Federal de Alagoas, Fazenda Sao Luiz s/n, Zona Rural de Vicosa, Vicosa, AL 57700-000, Brazil. E-mail: karla. ufal@gmail.com The objective of this study was to produce and partially purify Malleo-protein from Burkholderia mallei samples isolates from Equidae in Brazil with potential for use in the diagnosis of glanders. The strain B. mallei phenotypically characterized and proven virulent was inoculated into broth Dorset-Henley to grow and metabolize. The proteins were separated by trichloroacetic acid precipitation and amonium sulfate precipitation. The PPD mallein was concentrated 1.0mg/mL and biologically tested in guinea pigs. It was effective in the development of delayed-type hypersensitivity and consequently to identify true-positive animals and to exclude of true negatives. There is the possibility for potential use in the glanders diagnosis in Equidae.